GB格式文件和FASTA文件介紹
處理步驟
Python腳本代碼如下：
運行情況
GB格式文件和FASTA文件介紹在分子生物學中 我們會有將GB格式序列文件 轉換成 Fasta格式文件的需求，這裡我們利用python腳本來解決這個問題。
gb格式文件是GenBank的文件，用來保存序列的詳細信息。包含一個gene的名稱，編號，發現者，參考文獻，外顯子位置，編碼區序列，蛋白序列等等信息。
例如：
LOCUS NM_213806 849 bp mRNA linear MAM 24-SEP-2019DEFINITION Sus scrofa Fas ligand (TNF superfamily, member 6) (FASLG), mRNA.ACCESSION NM_213806VERSION NM_213806.1KEYWORDS RefSeq.SOURCE Sus scrofa (pig) ORGANISM Sus scrofa Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae; Sus.REFERENCE 1 (bases 1 to 849) AUTHORS Lin F, Fu YH, Han J, Shen M, Du CW, Li R, Ma XS and Liu HL. TITLE Changes in the expression of Fox O1 and death ligand genes during follicular atresia in porcine ovary JOURNAL Genet. Mol. Res. 13 (3), 6638-6645 (2014) PUBMED 25177944 REMARK GeneRIF: Data suggest forkhead box protein O1 (FoxO1) involvement in the regulation of TNF-related apoptosis-inducing ligand TRAIL and Fas ligand FasL expression during follicular atresia. Publication Status: Online-OnlyREFERENCE 2 (bases 1 to 849) AUTHORS Xie GH, Wang SJ, Wang Y, Zhang Y, Zhang HZ, Jin S, Wang QF, Liu ZC and Ge HL. TITLE Fas Ligand gene transfer enhances the survival of tissue-engineered chondrocyte allografts in mini-pigs JOURNAL Transpl. Immunol. 19 (2), 145-151 (2008) PUBMED 18503890 REMARK GeneRIF: the result indicates that the expression of FasL by chondrocytes is capable of inducing apoptosis of activated T cellsREFERENCE 3 (bases 1 to 849) AUTHORS Chang HW, Jeng CR, Lin CM, Liu JJ, Chang CC, Tsai YC, Chia MY and Pang VF. TITLE The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocyte apoptosis in vitro JOURNAL Vet. Microbiol. 122 (1-2), 72-82 (2007) PUBMED 17321702 REMARK GeneRIF: The expression of FAS and FAS ligand in splenic macrophages co-infected with porcine circovirus 2 and porcine reproductive and respiratory syndrome virus is reportedREFERENCE 4 (bases 1 to 849) AUTHORS Tayade C, Black GP, Fang Y and Croy BA. TITLE Differential gene expression in endometrium, endometrial lymphocytes, and trophoblasts during successful and abortive embryo implantation JOURNAL J. Immunol. 176 (1), 148-156 (2006) PUBMED 16365405REFERENCE 5 (bases 1 to 849) AUTHORS Bai L, Maedler K, Donath M and Tuch BE. TITLE Expression of Fas but not Fas ligand on fetal pig beta cells JOURNAL Xenotransplantation 11 (5), 426-435 (2004) PUBMED 15303979 REMARK GeneRIF: FasL was not detected on fetal pig pancreatic cells but could be induced on both beta and non-beta cells when the cells were treated with IL1beta. Erratum:[Xenotransplantation. 2016 Mar;23(2):171-2. PMID: 27106874]REFERENCE 6 (bases 1 to 849) AUTHORS Tsuyuki S, Kono M and Bloom ET. TITLE Cloning and potential utility of porcine Fas ligand: overexpression in porcine endothelial cells protects them from attack by human cytolytic cells JOURNAL Xenotransplantation 9 (6), 410-421 (2002) PUBMED 12371937REFERENCE 7 (bases 1 to 849) AUTHORS Motegi-Ishiyama Y, Nakajima Y, Hoka S and Takagaki Y. TITLE Porcine Fas-ligand gene: genomic sequence analysis and comparison with human gene JOURNAL Mol. Immunol. 38 (8), 581-586 (2002) PUBMED 11792426REFERENCE 8 (bases 1 to 849) AUTHORS Muneta Y, Shimoji Y, Inumaru S and Mori Y. TITLE Molecular cloning, characterization, and expression of porcine Fas ligand (CD95 ligand) JOURNAL J. Interferon Cytokine Res. 21 (5), 305-312 (2001) PUBMED 11429161COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB027297.1. ##Evidence-Data-START## Transcript exon combination :: AB027297.1, AF397407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01893940, SAMN01915393 [ECO:0000348] ##Evidence-Data-END##FEATURES Location/Qualifiers source 1..849 /organism="Sus scrofa" /mol_type="mRNA" /db_xref="taxon:9823" /chromosome="9" /map="9" gene 1..849 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /note="Fas ligand (TNF superfamily, member 6)" /db_xref="GeneID:396726" CDS 1..849 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /note="CD95 ligand; tumor necrosis factor (ligand) superfamily, member 6; fas antigen ligand" /codon_start=1 /product="tumor necrosis factor ligand superfamily member 6" /protein_id="NP_998971.1" /db_xref="GeneID:396726" /translation="MQQPFNYPYPQIFWVDSSATSPWASPGSVFPCPASVPGRPGQRR PPPPPPPPPPPPTLLPSRPLPPLPPPSLKKKRDHNAGLCLLVMFFMVLVALVGLGLGM FQLFHLQKELTELRESASQRHTESSLEKQIGHPNLPSEKKELRKVAHLTGKPNSRSIP LEWEDTYGIALVSGVKYMKGSLVINDTGLYFVYSKVYFRGQYCNNQPLSHKVYTRNSR YPQDLVLMEGKMMNYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESKTF FGLYKL" mat_peptide 1..390 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /product="ADAM10-processed FasL form. {ECO:0000250}" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)" mat_peptide 1..249 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /product="FasL intracellular domain. {ECO:0000250}" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)" misc_feature 244..249 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /experiment="experimental evidence, no additional details recorded" /note="Cleavage, by SPPL2A. {ECO:0000250}; propagated from UniProtKB/Swiss-Prot (Q9BEA8.1); cleavage site" misc_feature 247..309 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1); transmembrane region" misc_feature 388..393 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /experiment="experimental evidence, no additional details recorded" /note="Cleavage, by ADAM10. {ECO:0000250}; propagated from UniProtKB/Swiss-Prot (Q9BEA8.1); cleavage site" mat_peptide 391..846 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /product="Tumor necrosis factor ligand superfamily member 6, soluble form. {ECO:0000250}" /experiment="experimental evidence, no additional details recorded" /note="propagated from UniProtKB/Swiss-Prot (Q9BEA8.1)" misc_feature 553..555 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /experiment="experimental evidence, no additional details recorded" /note="N-linked (GlcNAc...) asparagine. {ECO:0000255}; propagated from UniProtKB/Swiss-Prot (Q9BEA8.1); glycosylation site" misc_feature 751..753 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /experiment="experimental evidence, no additional details recorded" /note="N-linked (GlcNAc...) asparagine. {ECO:0000255}; propagated from UniProtKB/Swiss-Prot (Q9BEA8.1); glycosylation site" misc_feature 781..783 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /experiment="experimental evidence, no additional details recorded" /note="N-linked (GlcNAc...) asparagine. {ECO:0000255}; propagated from UniProtKB/Swiss-Prot (Q9BEA8.1); glycosylation site" exon 1..351 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /inference="alignment:Splign:2.1.0" exon 352..397 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /inference="alignment:Splign:2.1.0" exon 398..454 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /inference="alignment:Splign:2.1.0" exon 455..849 /gene="FASLG" /gene_synonym="CD95-L; FASL; TNFSF6" /inference="alignment:Splign:2.1.0"ORIGIN 1 atgcagcagc ccttcaatta cccatacccc caaatcttct gggtggacag cagtgctacc 61 tctccctggg cctccccagg ctcagtcttc ccctgtccag cttctgtgcc aggaaggcca 121 gggcaaagga ggccaccacc accaccgccg ccaccgccac caccaccaac actcctgcca 181 tcaagaccgc tgcctccact gccaccgcca tctctgaaga agaagaggga ccacaatgca 241 ggcctgtgtc tccttgtgat gttcttcatg gttctggtgg ccctggttgg attggggctg 301 gggatgtttc agctcttcca cctacagaag gagctgactg aactcagaga gtctgccagc 361 caaaggcata cagaatcatc tttggagaag caaataggtc accccaatct accctctgag 421 aaaaaggagc tgagaaaagt ggcccactta acaggcaagc ctaactcaag atccatccct 481 ctggaatggg aagacaccta tggaattgcc ttggtctctg gggtgaagta tatgaagggc 541 agccttgtga tcaatgacac tgggctgtat tttgtgtatt ccaaagtgta cttccggggt 601 cagtactgca acaaccagcc cctgagtcac aaggtataca caaggaactc taggtatccc 661 caggacctgg tgctgatgga gggaaagatg atgaactatt gcactactgg ccaaatgtgg 721 gcccgcagca gctacctggg ggctgtgttc aatctcacca gcgctgacca tttatatgtc 781 aacgtatctg agctctctct ggtcaatttt gaggaatcta agacattttt tggcttatat 841 aagctctga//fasta格式是一種基於文本用於表示核酸序列或多肽序列的格式。其中核酸或氨基酸均以單個字母來表示，且允許在序列前添加序列名及注釋。該格式已成為生物信息學領域的一項標准。
例如：
處理步驟>NM_213806
ATGCAGCAGCCCTTCAATTACCCATACCCCCAAATCTTCTGGGTGGACAGCAGTGCTACC
TCTCCCTGGGCCTCCCCAGGCTCAGTCTTCCCCTGTCCAGCTTCTGTGCCAGGAAGGCCA
GGGCAAAGGAGGCCACCACCACCACCGCCGCCACCGCCACCACCACCAACACTCCTGCCA
TCAAGACCGCTGCCTCCACTGCCACCGCCATCTCTGAAGAAGAAGAGGGACCACAATGCA
GGCCTGTGTCTCCTTGTGATGTTCTTCATGGTTCTGGTGGCCCTGGTTGGATTGGGGCTG
GGGATGTTTCAGCTCTTCCACCTACAGAAGGAGCTGACTGAACTCAGAGAGTCTGCCAGC
CAAAGGCATACAGAATCATCTTTGGAGAAGCAAATAGGTCACCCCAATCTACCCTCTGAG
AAAAAGGAGCTGAGAAAAGTGGCCCACTTAACAGGCAAGCCTAACTCAAGATCCATCCCT
CTGGAATGGGAAGACACCTATGGAATTGCCTTGGTCTCTGGGGTGAAGTATATGAAGGGC
AGCCTTGTGATCAATGACACTGGGCTGTATTTTGTGTATTCCAAAGTGTACTTCCGGGGT
CAGTACTGCAACAACCAGCCCCTGAGTCACAAGGTATACACAAGGAACTCTAGGTATCCC
CAGGACCTGGTGCTGATGGAGGGAAAGATGATGAACTATTGCACTACTGGCCAAATGTGG
GCCCGCAGCAGCTACCTGGGGGCTGTGTTCAATCTCACCAGCGCTGACCATTTATATGTC
AACGTATCTGAGCTCTCTCTGGTCAATTTTGAGGAATCTAAGACATTTTTTGGCTTATAT
AAGCTCTGA
將文件夾下gb文件批量讀取
將各個gb文件中的登錄號和具體序列抽提出來，並寫入fasta文件
將fasta文件進一步處理去掉換行符，使一個完整的序列中沒有換行符
將所有處理好的fasta文件存入一個新建的子文件夾中
Python腳本代碼如下：#!/usr/bin/env python# -*- encoding: utf-8 -*-'''@File : [email protected] : 2020/07/04 14:15:[email protected] : [email protected] : [email protected] : [email protected]@License : (C)Copyright 2020 [email protected] : 將gb文件轉換為fasta文件，同時轉成無換行符，最後存入新的子文件夾中'''# here put the import libimport osimport shutildef gb2fasta(path): # 從gb文件中抽取登錄號和具體序列信息存入同名fasta文件 # 讀取文件夾相關信息 for root,dirs,files in os.walk(path): for file in files: # 打印文件所屬目錄 print(root + '\\' + file) # 獲取文件路徑 path_gb = os.path.join(root,file) flag=0 if path_gb[-2:] == 'gb': # 打開新建fasta文件准備寫入 fasta = open (path_gb[:-2]+'fasta','w') # 打開gb文件，准備讀取序列信息並寫入fasta文件 with open (path_gb,'r') as f: # 逐行掃描 for line in f: # 如果是ACCESSION行，則寫入fasta文件作為序列標題 if line[0:9] =='ACCESSION': fasta.writelines('>'+line.split()[1]+'\n') # 如果是ORGIN行，代表是序列 elif line[0:6] =='ORIGIN': flag = 1 elif flag == 1: #通過空格符（空格 換行 制表）對字符串進行切片 s = line.split() #非空切片字符打印 if s != []: #print(s) #去掉列表首個元素（數字序號）後，連接所有元素即為完整序列按行寫入fasta文件 seq=''.join(s[1:]) fasta.writelines(seq.upper()+'\n') fasta.close()def multi2single(path): #此函數功能為： 將多行序列轉換為單行序列（即去掉換行符），成為標准fasta文件 for root,dirs,files in os.walk(path): for file in files: path_full = os.path.join(root,file) # 有fasta 且不為 single.fasta，才進行單行轉換，否則會重復創建文件夾 a = path_full[-5:] == 'fasta' if path_full[-12:] == 'single.fasta': b = True else: b = False b = bool(1-b) if a & b : fr=open(path_full, 'r') fw=open(path_full[:-6]+'_single.fasta', 'w') seq={} for line in fr: if line.startswith('>'): #判斷字符串是否以‘>開始' name=line.split()[0] #以空格為分隔符，並取序列為0的項。 seq[name]='' else: seq[name]+=line.replace('\n', '') fr.close() for i in seq.keys(): fw.write(i) fw.write('\n') fw.write(seq[i]) fw.write('\n') fw.close()def copy2subdir(path): # 將生成的_single.fasta文件存入新的子文件夾.\singl_fasta\中 root1 = path for root,dirs,files in os.walk(path): subdir = os.path.join(root1,'singl_fasta\\') for file in files: oldfile = os.path.join(root1,str(file)) newfile = os.path.join(root1,'singl_fasta\\',str(file)) if not os.path.exists(subdir): os.makedirs(subdir) print("目錄創建成功！") if oldfile[-12:] == 'single.fasta': if not os.path.exists(newfile): shutil.copyfile(oldfile , newfile) print("\n你的fasta文件保存在: " + subdir + "文件夾下\n")if __name__ == "__main__": path = input("請輸入路徑") #此處輸入 D:\docu\gb2fasta gb2fasta(path) multi2single(path) copy2subdir(path)運行情況PS D:\vscode_python_magic> & d:/ruanjiancangku/python_projectkotin/venv/Scripts/python.exe d:/vscode_python_magic/實驗室-magic/序列處理/gb2fasta.pyD:\docu\gb2fasta\1-FASLG-swine-849bp-NM_213806.fastaD:\docu\gb2fasta\1-FASLG-swine-849bp-NM_213806.gb['1', 'atgcagcagc', 'ccttcaatta', 'cccatacccc', 'caaatcttct', 'gggtggacag', 'cagtgctacc']['61', 'tctccctggg', 'cctccccagg', 'ctcagtcttc', 'ccctgtccag', 'cttctgtgcc', 'aggaaggcca']['121', 'gggcaaagga', 'ggccaccacc', 'accaccgccg', 'ccaccgccac', 'caccaccaac', 'actcctgcca']['181', 'tcaagaccgc', 'tgcctccact', 'gccaccgcca', 'tctctgaaga', 'agaagaggga', 'ccacaatgca']['241', 'ggcctgtgtc', 'tccttgtgat', 'gttcttcatg', 'gttctggtgg', 'ccctggttgg', 'attggggctg']['301', 'gggatgtttc', 'agctcttcca', 'cctacagaag', 'gagctgactg', 'aactcagaga', 'gtctgccagc']['361', 'caaaggcata', 'cagaatcatc', 'tttggagaag', 'caaataggtc', 'accccaatct', 'accctctgag']['421', 'aaaaaggagc', 'tgagaaaagt', 'ggcccactta', 'acaggcaagc', 'ctaactcaag', 'atccatccct']['481', 'ctggaatggg', 'aagacaccta', 'tggaattgcc', 'ttggtctctg', 'gggtgaagta', 'tatgaagggc']['541', 'agccttgtga', 'tcaatgacac', 'tgggctgtat', 'tttgtgtatt', 'ccaaagtgta', 'cttccggggt']['601', 'cagtactgca', 'acaaccagcc', 'cctgagtcac', 'aaggtataca', 'caaggaactc', 'taggtatccc']['661', 'caggacctgg', 'tgctgatgga', 'gggaaagatg', 'atgaactatt', 'gcactactgg', 'ccaaatgtgg']['721', 'gcccgcagca', 'gctacctggg', 'ggctgtgttc', 'aatctcacca', 'gcgctgacca', 'tttatatgtc']['781', 'aacgtatctg', 'agctctctct', 'ggtcaatttt', 'gaggaatcta', 'agacattttt', 'tggcttatat']['841', 'aagctctga']['//']D:\docu\gb2fasta\2-LTA-swine-1584bp-NM_214453.fastaD:\docu\gb2fasta\2-LTA-swine-1584bp-NM_214453.gb['1', 'agaaaggggc', 'ccacaggggt', 'cccgcacagc', 'aggtgagact', 'ctcccacccc', 'atctcctagg']['61', 'gctgtccggg', 'tgctggactc', 'ccccctcact', 'tcggtccctc', 'cgcccgctcc', 'ctggccttcc']['121', 'tgcccctcct', 'gcatcttcac', 'cccggcctgg', 'gccttggtgg', 'gtttggtttt', 'ggtttgttct']['181', 'ctctgattct', 'ttatctgtca', 'ggctctttct', 'agctctcaca', 'cactctgatc', 'cctctctgtt']['241', 'cccttcccat', 'ctctgtttct', 'ctctgggtct', 'ccccctgctc', 'acctcgggat', 'ttccctgagt']['301', 'gcctctggtc', 'cccttctctg', 'tctggcgccc', 'cgtctcttgt', 'ctctcggggt', 'ggctgtctcc']['361', 'gagggcagga', 'ggccttcttc', 'cgcaggtgcc', 'ccgccccgct', 'cactgtctct', 'ctccccccac']['421', 'aggttttccc', 'catgacacca', 'cctggacgcc', 'tctacctccg', 'gagggtgtgc', 'agcaccccca']['481', 'tcctcctcct', 'cctggggctg', 'ctgctggccc', 'tgccgcccga', 'ggcccagggg', 'ctccctggcg']['541', 'tcggcctccc', 'accctcagct', 'gcacagcctg', 'cccatcagca', 'ccccccaaag', 'cacttggcca']['601', 'gaggcaccct', 'caaacctgcc', 'gctcacctcg', 'ttggagaccc', 'cagcaccccg', 'gactcactgc']['661', 'gctggagagc', 'gaacacggat', 'cgtgccttcc', 'tccgccatgg', 'cttcttgctg', 'agcaacaact']['721', 'ccctgctggt', 'ccccaccagt', 'ggcctctact', 'ttgtctactc', 'ccaggtcgtc', 'ttctccgggg']['781', 'aaggctgctt', 'ccccaaggcc', 'acccccaccc', 'ctctctacct', 'ggcccacgag', 'gtccagctct']['841', 'tctcctccca', 'gtaccccttc', 'cacgtgccgc', 'tcctcagcgc', 'tcagaagtcc', 'gtgtgccccg']['901', 'ggccacaggg', 'accttgggtg', 'cgctctgtgt', 'accagggggc', 'tgtgttcctg', 'ctcacccagg']['961', 'gagatcagct', 'gtccacacac', 'acagacggca', 'ccccccacct', 'gctcctcagc', 'cccagtagcg']['1021', 'tcttctttgg', 'agccttcgct', 'ctatagaaga', 'atccagaaag', 'aaaaaaattg', 'gtttcaaggc']['1081', 'cttctcccct', 'tttcacctcc', 'cttatgacca', 'cttcggaggt', 'caccgcgcct', 'ctcctctgac']['1141', 'aatttccaac', 'agtctcatct', 'tcccccacgc', 'tcagcacctg', 'gagcttctgt', 'agaaggaatt']['1201', 'ctaggcacct', 'cgggggaact', 'ggaaccaccc', 'cggatgctct', 'gctgaggatc', 'tgaatgcccg']['1261', 'cctggagccc', 'ttcccctgtc', 'ctgcccgtct', 'aggggccctc', 'gtccaggacg', 'tggaagggaa']['1321', 'gctgacccat', 'gagggacttt', 'gaacggatga', 'ccggagcggt', 'gtgggggggt', 'tatttatgaa']['1381', 'ggggaaaatt', 'aaattattta', 'tttatggagg', 'atggagagaa', 'gggaatcaca', 'gagggatgtc']['1441', 'agaagagtgt', 'gacacatgtg', 'cccaagagat', 'aaagtgacag', 'aaggcatggg', 'ctccagatga']['1501', 'cccggccaga', 'gagggcaaag', 'tggctcagga', 'aggggctgct', 'tgactggagg', 'ctcatgagga']['1561', 'gacggctgac', 'cctcgatgaa', 'accc']['//']D:\docu\gb2fasta\3-LTB-swine-950bp-NM_001185138.fastaD:\docu\gb2fasta\3-LTB-swine-950bp-NM_001185138.gb['1', 'tcggatgggg', 'gcaccggggc', 'tggagggccg', 'gggtaggagg', 'ccccagggga', 'agggatgcct']['61', 'cctgctggcc', 'gtggcagggg', 'ccacttccct', 'ggtgaccctc', 'ctgctggccg', 'tgcctatcac']['121', 'ggtcctggct', 'gtgctggcct', 'tggtgcccca', 'ggagcaggga', 'gaactggtaa', 'cagggaccgc']['181', 'tgacccaggc', 'acccaggcgg', 'aggcccagca', 'gcgattggag', 'tccaaggaga', 'cgccagagga']['241', 'ggaggcagaa', 'acagatctca', 'gccccaggct', 'cccagctgcc', 'cacctcattg', 'gcgcttggat']['301', 'cacgggtcag', 'gggctaggct', 'gggaggcgaa', 'gaaagaagag', 'gcgtttctga', 'ggagcgggac']['361', 'gcagttctct', 'ggcgcggagg', 'gcctggccct', 'cccgcaggac', 'ggcctctact', 'acctctactg']['421', 'tcacgtcggc', 'taccggggcc', 'gggcacctcc', 'tcccggcggg', 'gaccccctgg', 'accgctcggt']['481', 'cacgctgctc', 'agccggctgt', 'accgggcggg', 'gggcgcctac', 'ggaccgggga', 'ctcccgagct']['541', 'gctgctggag', 'ggcgcggaga', 'ctgtgactcc', 'ggtcttggac', 'cccagtcgga', 'ggcacgagta']['601', 'cgggcccctc', 'tggtacacga', 'gcgtggggtt', 'cggtggcctg', 'gtgcagctcc', 'ggaggggcga']['661', 'gagggtgtac', 'gttaatatca', 'gtcaccccga', 'tatggtggat', 'tacaggagag', 'gaaagacctt']['721', 'cttcggggcg', 'gtgatggtgg', 'gctgaggact', 'gtccgcggcc', 'cgagaggacc', 'actgcatggt']['781', 'gggagtgtgt', 'cgatggatca', 'agcccagaca', 'cggggtccca', 'gacaccaggc', 'cagacaccat']['841', 'ggccgtgggg', 'aaaatgcagg', 'agatcgtgtg', 'gaaaactgat', 'tttgagcctg', 'atgaaaataa']['901', 'agaatgtaaa', 'agctttaatn', 'gctgcccatg', 'ccaaaaaaaa', 'aaaaaaaaaa']['//']D:\docu\gb2fasta\4-TNF-swine-1666bp-NM_214022.fastaD:\docu\gb2fasta\4-TNF-swine-1666bp-NM_214022.gb['1', 'cccagagtga', 'ggacaccagg', 'ggaccagcca', 'ggagagagac', 'aagccatctc', 'caggaccccc']['61', 'tagaaataac', 'ctctcagaag', 'acacaccccc', 'gaacaggcag', 'ccggacgact', 'ctctccctct']['121', 'cacacgctgc', 'cccggggcgc', 'caccatctcc', 'cagctggacc', 'tgagcccctc', 'tgaaaaagac']['181', 'accatgagca', 'ctgagagcat', 'gatccgagac', 'gtggagctgg', 'cggaggaggc', 'gctcgccaag']['241', 'aaggccgggg', 'gcccccaggg', 'ctccaggagg', 'tgcctgtgcc', 'tcagcctctt', 'ctccttcctc']['301', 'ctggtcgcag', 'gagccaccac', 'gctcttctgc', 'ctactgcact', 'tcgaggttat', 'cggcccccag']['361', 'aaggaagagt', 'ttccagctgg', 'ccccttgagc', 'atcaaccctc', 'tggcccaagg', 'actcagatca']['421', 'tcgtctcaaa', 'cctcagataa', 'gcccgtcgcc', 'cacgttgtag', 'ccaatgtcaa', 'agccgaggga']['481', 'cagctccaat', 'ggcagagtgg', 'gtatgccaat', 'gccctcctgg', 'ccaacggcgt', 'gaagctgaaa']['541', 'gacaaccagc', 'tggtggtgcc', 'gacagatggg', 'ctgtacctca', 'tctactccca', 'ggtcctcttc']['601', 'aggggccaag', 'gctgcccttc', 'caccaacgtt', 'ttcctcactc', 'acaccatcag', 'ccgcatcgcc']['661', 'gtctcctacc', 'agaccaaggt', 'caacctcctc', 'tctgccatca', 'agagcccttg', 'ccagagggag']['721', 'acccccgagg', 'gggccgaggc', 'caagccctgg', 'tacgaaccca', 'tctacctggg', 'aggggtcttc']['781', 'cagctggaga', 'aggatgatcg', 'actcagtgcc', 'gagatcaacc', 'tgcccgacta', 'tctggacttt']['841', 'gctgaatctg', 'ggcaggtcta', 'ttttgggatc', 'attgccctgt', 'gagggggcag', 'gacatccgtt']['901', 'ccctcccctg', 'tccatccctt', 'tattatttta', 'ctccttcaga', 'ccccctcacg', 'tccttctggt']['961', 'ttagaaagag', 'aatgaggggc', 'tggggactgg', 'gctccaagct', 'taaaacttta', 'aacaacaaca']['1021', 'gcaacactta', 'gaaatcaggg', 'attcagggat', 'gtgtggcctg', 'gacaaccagg', 'cactgaccac']['1081', 'caccaagaat', 'tggaactggg', 'gcttccagac', 'tcgctggggt', 'ccttgggttt', 'ggattcctgg']['1141', 'atgcaacctg', 'ggacatctgg', 'aatgtggctg', 'ccagggaagc', 'ttgggttcca', 'atcggaatac']['1201', 'ttcagaacat', 'tccttgagaa', 'gatttcacct', 'caatcttgat', 'gactttttag', 'gcttcccttt']['1261', 'cttccaattt', 'tccagacttc', 'cctgggatgg', 'ggagcccagc', 'cccaaacccc', 'acaggccagc']['1321', 'tccctcttat', 'ttatatttgc', 'acttggcatt', 'attatttatt', 'tatttattta', 'ttatttattt']['1381', 'actagtgaat', 'gtatttattc', 'aggagggcga', 'ggtgtcctgg', 'gagacccagc', 'ataagggctg']['1441', 'ccttggttca', 'gatgtgtttt', 'ctgtgaaaac', 'ggagctgaac', 'tgtaggttgc', 'tcccacctgg']['1501', 'cctcctagcc', 'tctgtgcctc', 'cttttgctta', 'tgtttttaaa', 'aacaaatatt', 'tatctgatcg']['1561', 'agttgtctaa', 'ataatgctga', 'tttggtgact', 'aacttgtcgc', 'tacatcgctg', 'aacctctgct']['1621', 'ccccagggga', 'gttgtgtctg', 'taaccgccct', 'actggtcagt', 'ggcgag']['//']D:\docu\gb2fasta\5-TNFSF4-swine-549bp-NM_001025217.fastaD:\docu\gb2fasta\5-TNFSF4-swine-549bp-NM_001025217.gb['1', 'atggaagggg', 'tccaacccct', 'agatgaaaat', 'gtgggaaacg', 'caccaggacg', 'aagactcttg']['61', 'aggaacaagc', 'tattgttggt', 'ggcctccgta', 'attcagggtc', 'tggggttgct', 'cctgtgtctc']['121', 'acctacatct', 'gcctgcacct', 'ctatgctcag', 'gtgccatctc', 'agtaccctcc', 'aattcagagt']['181', 'atcaaagtac', 'aatttaccaa', 'gtgtgaaaat', 'gataatggtt', 'tcatcatcac', 'accctcaagc']['241', 'aaggatggaa', 'ccatgaaagt', 'gcaaaacaac', 'tcaatcatca', 'tcaactgtga', 'tgggttctat']['301', 'ctcatctccc', 'tgaagggtta', 'cttttctcag', 'gagctcagcc', 'tcatgcttca', 'gtaccggaag']['361', 'ggtcggaaac', 'ctctcttctc', 'cctgaacaag', 'gtcaagtctg', 'tggactctgt', 'cacagtagcc']['421', 'gatctggctt', 'tcaaggacaa', 'ggtcttcctg', 'aacgtgacca', 'ctcatagtgc', 'ctcctgtgaa']['481', 'gacattcagg', 'tgaatggtgg', 'ggaattgatt', 'ctcattcatc', 'aaaatcctgg', 'tggattctgt']['541', 'gtctactga']['//']D:\docu\gb2fasta\6-TNFSF10-swine-1696bp-NM_001024696.fastaD:\docu\gb2fasta\6-TNFSF10-swine-1696bp-NM_001024696.gb['1', 'agcagtcaga', 'ccctgcctgg', 'accatggcgg', 'tgatgcagac', 'tccaggaggc', 'cccagccccg']['61', 'ggcagacctg', 'tgtgttgatc', 'ctgatcttca', 'cagtgctcct', 'gcaagccctc', 'tgtgtggcct']['121', 'tgacttacgt', 'gtacttcacc', 'aatgaactga', 'aacagatgca', 'ggacaagtac', 'tccaaaagcg']['181', 'gtatagcttg', 'cttcttaaag', 'gaagatgaca', 'gtttctggga', 'tcccaccgat', 'gacgagagaa']['241', 'tgctcagccc', 'ctgctggcag', 'gtgaagtggc', 'agctacgtca', 'gtttgtgaga', 'aagatgattt']['301', 'tgagaaccta', 'tgaggaaacc', 'atttctacag', 'tttcagaaaa', 'gcaacaaggc', 'attcctcacc']['361', 'tagaaagaga', 'aaaaggtcca', 'cagagagtgg', 'ctgctcacat', 'aactggaacc', 'agtaggaaaa']['421', 'gaagcacatt', 'tccatctcta', 'agctccaaat', 'atgaaaaagc', 'tttgggccag', 'aaaataaact']['481', 'cctgggaatc', 'atcaagaaaa', 'ggacattcat', 'tcttgaataa', 'ttttcacttg', 'aggaatggag']['541', 'agctggttat', 'ccatcaaaca', 'gggttttact', 'acatctattc', 'ccaaacatac', 'tttcgatttc']['601', 'aggaacctga', 'ggaaattttg', 'ggaacggttt', 'ctacagaagg', 'gaacagaaag', 'aaaaacaggc']['661', 'aaatgataca', 'gtatatttac', 'aaatggacaa', 'gctatcctga', 'ccctatactg', 'ctgatgaaaa']['721', 'gtgctagaaa', 'tagttgttgg', 'tctaaagatt', 'cagaatatgg', 'actctattcc', 'atctatcaag']['781', 'gtggaatatt', 'tgagcttaag', 'gaagatgacc', 'gaatttttgt', 'ctctgttact', 'aatgagcaac']['841', 'tgattgacat', 'ggaccaagaa', 'gccagttttt', 'tcggggcctt', 'tttaattggc', 'taaatgatct']['901', 'gcagggaaaa', 'aaaccatgcc', 'ccagagtgac', 'tattcagagt', 'cgtatactgt', 'gaaaatattc']['961', 'cagcagagcc', 'aataggttaa', 'ggcagcctga', 'gcaaagaggc', 'ctcaacccaa', 'aggctcaaca']['1021', 'acacaagctt', 'tttggaaagt', 'gaaaagtgac', 'caattccttc', 'caggaaaatg', 'aaactgccaa']['1081', 'gagaccttgt', 'ggagctctgc', 'ctgatgtcat', 'tttgctagta', 'aacatctaga', 'agatactctg']['1141', 'tctccaaatt', 'tgtgtaacaa', 'ttaacacctc', 'ctgcctttat', 'catctaatcc', 'tgtgaagatt']['1201', 'ctagaagaaa', 'gagtagtgat', 'ccatctcagg', 'tgggaataag', 'ggacaacatt', 'cccaaaacta']['1261', 'aagagaaaag', 'ggcagcactg', 'aaaggtcaca', 'gtcaatatat', 'gcagtttcag', 'tacaaacata']['1321', 'acaaattaaa', 'gctacgttta', 'gtggacaagg', 'agctacttct', 'gaatggtttg', 'tgcttttctc']['1381', 'tactaaaaat', 'caggctggcc', 'aaaagcactc', 'agggtatttt', 'tgataaagga', 'ctctaaaata']['1441', 'agtgataaag', 'tatggcgata', 'cctcagaaaa', 'ctaaatacag', 'aactaccaca', 'tgacccagca']['1501', 'atcccactcc', 'tgtgcatata', 'tctggacaaa', 'actttccttg', 'aaaaagatac', 'attcatctct']['1561', 'atgctcattg', 'cagcactatt', 'cacagtagcc', 'aagacatgga', 'aacacctata', 'tgtctatgaa']['1621', 'tggatgaata', 'gattaagaag', 'gtgtgttatg', 'tatacatant', 'ggaatactgg', 'gaagccataa']['1681', 'aaaggacaaa', 'gaggcc']['//']D:\docu\gb2fasta\7-TNFSF13b-swine-1013bp-NM_001097498.fastaD:\docu\gb2fasta\7-TNFSF13b-swine-1013bp-NM_001097498.gb['1', 'tctaggaggg', 'aaatggatga', 'ctccacgggg', 'gagcagtcac', 'gcctttcttg', 'ccttagcacg']['61', 'agagaagaaa', 'tgaaactgaa', 'ggagacggtc', 'cccatcctcc', 'cccagaagga', 'aagcccctct']['121', 'gtccgcatct', 'ccaaagatgg', 'gaagctgctg', 'gtcgtgaccc', 'tgctgctggc', 'cctgctgtcc']['181', 'tgctgcctca', 'cggggatctt', 'tgcaccacca', 'gctccaaggg', 'agagcagctc', 'cattcaaagc']['241', 'aacaggagta', 'agcgcgccgc', 'gcaggatgcg', 'gaggagacag', 'tcactcagga', 'ctgcttgcaa']['301', 'ttgattgcag', 'acagtgacat', 'gcctactata', 'cgaaaaggag', 'cttatacatt', 'tgttccatgg']['361', 'cttctcagct', 'ttaaaagagg', 'aagagcccta', 'gaagaaaaag', 'aaaataaaat', 'cgtggtcaaa']['421', 'gaaacgggtt', 'acttttttat', 'atacggtcag', 'gttttataca', 'ccgataacac', 'ctttgccatg']['481', 'gggcatctca', 'tacagaggaa', 'gaaagtccat', 'gtctttgggg', 'atgaactgag', 'tctggtgact']['541', 'ttgttccgat', 'gtattcaaaa', 'tatgcctgaa', 'acactaccca', 'ataattcctg', 'ttattcagct']['601', 'ggcattgcaa', 'agctggagga', 'aggagatgaa', 'ctccaactgg', 'caataccacg', 'tgaagacgct']['661', 'aaaatatcac', 'gggatggaga', 'cggcacattt', 'tttggtgcat', 'tgaaacttct', 'gtgacctact']['721', 'tacaccttgt', 'ttgtggctct', 'tgccctccct', 'ccctctgtac', 'ctctaaagag', 'aaaacactta']['781', 'actggaaata', 'ccaaaagggg', 'aaaaaaaagt', 'agttaccata', 'gccttttctg', 'tgagctgttt']['841', 'gttttggttt', 'gctgaaacta', 'gaccaaaaca', 'ggaaatttaa', 'cagacaacca', 'cagccaaagg']['901', 'gtatcatgtg', 'aattacaaga', 'aatagagccc', 'atttaagaaa', 'aaatagaatt', 'agaaagactt']['961', 'ttcactgtaa', 'tgccatgttg', 'aacagcttag', 'tcatagcttc', 'ttgtcttgga', 'gga']['//']D:\docu\gb2fasta\8-TNFSF18-swine-5005bp-XM_005667782.fastaD:\docu\gb2fasta\8-TNFSF18-swine-5005bp-XM_005667782.gb['1', 'gcattccatt', 'taacaaatga', 'aaaggctaag', 'gcataaagaa', 'ccaggagaga', 'gaaccggaga']['61', 'tttcctcaat', 'tttagtgtag', 'taaatcaaca', 'gttttagtgc', 'taggaagttt', 'tttggaatag']['121', 'agtgtaaact', 'cagatggtag', 'gacagggtgc', 'atgaaaatat', 'ccttttctat', 'gataacttta']['181', 'tttctgtctg', 'atgtcagcat', 'tgaaatttca', 'gagtattaaa', 'atggtgaggt', 'atgaaaaaac']['241', 'taagcttgtt', 'gttattgaca', 'tatttttaaa', 'aataaaattc', 'tagtaataca', 'ttactgtttt']['301', 'ctagagatta', 'tctcagaatg', 'gacacattgt', 'ataccctagc', 'aattgatgaa', 'aatatttttc']['361', 'ccaaaccatg', 'aaccccagca', 'tccttagctc', 'cctgacctac', 'tgcctcccac', 'aaacatgata']['421', 'tttggagtat', 'gatagacctt', 'catcttgaat', 'ttcattcttt', 'ttcttacaaa', 'agtaattttc']['481', 'ttatctggaa', 'ataatttgta', 'atgttgaata', 'gttccatagt', 'tcctcttgct', 'tcagaaaata']['541', 'tttatttttc', 'ctcttaactt', 'cccttgttgg', 'gttttttttt', 'tttaaatcat', 'ctgtgtttgt']['601', 'gttggcttct', 'agccctcagt', 'tccagcacct', 'ttggtctggt', 'gccaaatgtt', 'agtcagcact']['661', 'taggctaaaa', 'gtatcgtttt', 'ccaacaccca', 'gatcagaagg', 'aaaactccgc', 'ctcttacacc']['721', 'cactacttag', 'tgctatacta', 'caaaactgac', 'tagttgaatc', 'atgtgctcat', 'tacttctgaa']['781', 'tttctgcttt', 'tcacaactct', 'cattcctgca', 'gagaatgagt', 'ttgagccaca', 'tggagaatat']['841', 'gcctttaagc', 'cattcaagtc', 'ctcacgcagc', 'acagagacca', 'tcctggaagc', 'aatggctact']['901', 'ctactcaaca', 'atagttattt', 'tgctattact', 'ttgctccttc', 'agtgcactaa', 'tcttaacttt']['961', 'tctcccactc', 'aagacctcca', 'acaggccatg', 'tgtagcgaag', 'tttggaccat', 'taccttcaaa']['1021', 'atggcaaatg', 'ccatctcctg', 'agccttcttg', 'tgtgaataag', 'acagatgatt', 'ggaggctgaa']['1081', 'gatacttcag', 'aatggcttgt', 'atttaattta', 'tggccaagtg', 'gctcccaaca', 'cagcttacaa']['1141', 'ggggcaagct', 'ccttttgagg', 'tgttgctacg', 'taggaatgaa', 'gaccccatac', 'aatctctaac']['1201', 'gaacaattct', 'acagtccaga', 'atgtaggagg', 'ggcttatgaa', 'tttcatgctg', 'gagatgtaat']['1261', 'agacttgata', 'ttcaatgctg', 'aacatcaggt', 'tctaaaaaat', 'aatacatact', 'gggggatctt']['1321', 'tctgctagca', 'aatccccaat', 'tcatctccta', 'gagactcagt', 'taggtctcct', 'catcttcagc']['1381', 'acatgcagag', 'atgccagtgc', 'ataggatgga', 'gaaggaagat', 'tttcaacaca', 'tacagttcat']['1441', 'ctgggtatac', 'aaatcaacat', 'gaacagatct', 'cctctgcatg', 'tgaagcttca', 'tttctcctgc']['1501', 'ttattgaatg', 'agactcagaa', 'agcactgaag', 'acatttggtt', 'acccctgatg', 'ttgggtcagc']['1561', 'aaagacactt', 'tactagttca', 'tgataaaatg', 'aaaatgggtg', 'gctggaagac', 'aaaatctttt']['1621', 'caaagtgtct', 'gtctaatcct', 'tgaacccctg', 'agtggaaaaa', 'tgaggtctat', 'tcccataata']['1681', 'gccttatata', 'gcatgcaaaa', 'aaagaccagg', 'gcagtagcct', 'ggtcttgttc', 'ttatattctt']['1741', 'ggactgtgga', 'ctgtttcaat', 'tcattcttcc', 'catattctca', 'tcttaggaga', 'cactcttaat']['1801', 'aaaatgtagt', 'cagagtgggt', 'gtgtggccag', 'caacactcca', 'ttttggagtt', 'gatgagatta']['1861', 'ggggatagag', 'aacactctta', 'ggaaatattg', 'ggacagaatt', 'tcagttggca', 'ttgaaatgga']['1921', 'atgcacttta', 'ttcgggaatt', 'tcacttgatt', 'tcatcatcaa', 'gtgcagggtg', 'ctctataaaa']['1981', 'cctgctggtc', 'aaaaggctag', 'ctttcaatct', 'tcacatagca', 'gttcatgaga', 'atttactggt']['2041', 'gtatgtatct', 'aaccatgtca', 'atgacaaaga', 'gtaatcatta', 'gtagtaagat', 'ctaacccccc']['2101', 'aaattggtat', 'taccagtact', 'gtactttgca', 'actgtgcaga', 'gccagctaaa', 'aatatgaaat']['2161', 'cattacatga', 'caaagcactt', 'tcatatacca', 'catggcaact', 'cgatagattt', 'aatggggcag']['2221', 'atatttttgg', 'cacaatttta', 'actatgagaa', 'tacagaggca', 'gatggaatag', 'aggtaacttg']['2281', 'gttcagttca', 'tagaactagt', 'aattaacagg', 'cacctgggct', 'tcccactgta', 'ttatactata']['2341', 'ttagctttac', 'gattggtatt', 'tctgctatca', 'tgttagaagc', 'ctataaactt', 'taacagattt']['2401', 'aaaattttca', 'gacagtatat', 'tcccttttag', 'tccaacagca', 'attttttcct', 'ttctcagcaa']['2461', 'atttcttttc', 'ttttctttgc', 'ctggagcagg', 'gtacccaggg', 'tgttattcaa', 'gacttactac']['2521', 'aacttaatct', 'ccttccttac', 'tttggtcaaa', 'tgtgttaact', 'tccaaaaata', 'atgaataata']['2581', 'ctcaattcag', 'ggacagtctg', 'ttaaattttt', 'ggactctgca', 'aaattaacta', 'gctgcttatg']['2641', 'ggttgttatt', 'aaaaggtatg', 'taggtaatgt', 'gattacatga', 'aaacccaatt', 'taaaatattt']['2701', 'atggatattt', 'gtaaaaaatc', 'tacattatgt', 'taattaatag', 'tatcaccatt', 'aaaaactaat']['2761', 'ttaagaatat', 'ttgtattgta', 'tgtaagaaaa', 'actgcttgga', 'agcagactaa', 'gcctgaggcc']['2821', 'aagatgcctc', 'atagtatgtc', 'tttttttttt', 'tttttttaaa', 'tacatctgct', 'gagcagctgt']['2881', 'agggacaaag', 'actggggtac', 'ctggttcctc', 'ttgtatttgt', 'gtatcatctc', 'aggaaattaa']['2941', 'agttacataa', 'catacatata', 'tttatggaaa', 'cgtggtattg', 'atgttaactt', 'ataagcagta']['3001', 'gtgtgctgga', 'gtgggctagc', 'actagctcag', 'gagagctgtt', 'aaatttttat', 'taattgtgta']['3061', 'gtctggttat', 'taaatcatta', 'tccttgaaat', 'tggccatggt', 'aggacaattt', 'ataccatgtg']['3121', 'aattagcaaa', 'tgctacaaat', 'cagggctttt', 'ctttttggaa', 'agcccatgca', 'ccagcacacc']['3181', 'actgtttata', 'aaactcttct', 'taatgactcc', 'tctcagcccc', 'tgcctcagta', 'ttacaacagt']['3241', 'caaggcaggc', 'aaggaaagtg', 'tcttactctc', 'agcaaaagcc', 'ccacagataa', 'atcatttctc']['3301', 'agggcaggtg', 'gaggaatcta', 'cagctgtaac', 'cagatagata', 'gctaccaaca', 'tatgaccttt']['3361', 'gaatttccct', 'agtgttgaaa', 'tttcaggctt', 'tgttttcaat', 'gtatactctg', 'ttcccttgtt']['3421', 'tcttcaaaac', 'agtgtttata', 'ttttaaactg', 'acaataaaat', 'gtttgtacat', 'gggctgtagc']['3481', 'tgatttatct', 'atgggttatc', 'tgacaagagt', 'tagtgatcta', 'gagaatgtgc', 'atcagagaaa']['3541', 'tccttgtcca', 'tatgaaacac', 'tgctgagaga', 'gtgagaggca', 'aaagtcagag', 'ctgggggttt']['3601', 'tgtcccaatt', 'tctgatgggt', 'taggcaaata', 'atttcactag', 'ctgactttca', 'cccacctcct']['3661', 'caactctacc', 'ccacccatcc', 'accctcacca', 'tcaaagccat', 'catcaaccct', 'ctcatactaa']['3721', 'ttgtttttac', 'cttctatttt', 'gcctcatcaa', 'agctgacctg', 'gagtgctttt', 'tatcactgtg']['3781', 'ggttaagtct', 'ctacttctga', 'agatgacagt', 'taattgatca', 'cagtcctgtg', 'tatttgatgg']['3841', 'aacagataaa', 'ctccttagat', 'gcattatatc', 'tttgcaactt', 'ggagataaaa', 'ttcctgtctt']['3901', 'tgtgtgttct', 'tcctcaaaga', 'agcaattttc', 'attggctggg', 'ggtgctagta', 'cgttttttat']['3961', 'tagtgagaag', 'gagaagcgaa', 'aacccaattg', 'acttatgggc', 'tagtggtctg', 'aggcttacct']['4021', 'tcaagcagag', 'tttttcaaag', 'tgctatctat', 'ggactctaca', 'ttagatttgc', 'ccagtatact']['4081', 'tggccaaaaa', 'ccaaagaaaa', 'accccaaaga', 'taccttgacc', 'tcattcatag', 'tccactaaat']['4141', 'aaatgtttct', 'aggagtgagg', 'tctaggaatc', 'aaatttgtaa', 'tgagctctga', 'cataggttat']['4201', 'tcttatacta', 'cagtttaaaa', 'accactgctt', 'tatggaatgc', 'aattttgaat', 'ctcagcagaa']['4261', 'tgtttctcca', 'aatatttaaa', 'aacagtcaaa', 'gcttataaac', 'caatgaagtt', 'aaaaagcaaa']['4321', 'gcaaaaagca', 'aaaccaaaat', 'tggtattcat', 'gagacttacc', 'ataagccatc', 'tctcatcaca']['4381', 'aacaatggcc', 'ttctatcctc', 'tatttcagtt', 'tgaaaaaaag', 'gtcaggctct', 'tcactttcca']['4441', 'taatctaatg', 'gcaactttgc', 'cagttgaatg', 'ctggcaatcc', 'atagcatatg', 'atccttgtca']['4501', 'taatcagtac', 'tccattatct', 'acctgtatgc', 'tgtcaactct', 'caaatactta', 'tctctagcca']['4561', 'aagttgttct', 'cctttaagtc', 'ttataactcc', 'acctgtctgc', 'acagtaattt', 'cacattggac']['4621', 'atacaatgga', 'catctcaaat', 'tcaacttacc', 'ccaaatgaat', 'cttttaaggt', 'ttcaactaaa']['4681', 'gcagcttttt', 'tcgaatccct', 'cctcatttca', 'attgtatctc', 'atgagccaaa', 'aacctctgaa']['4741', 'tcatggagtc', 'attcctgact', 'tctatcttta', 'atctgtttaa', 'tcttaggaaa', 'actattatct']['4801', 'ttactttcag', 'agaatctgac', 'ctctttttgg', 'ctatggctct', 'tgtctttgga', 'ccaatatttt']['4861', 'tttcagtatc', 'ttcataactt', 'attacagtat', 'ctacatactt', 'attacattca', 'ggatttttgg']['4921', 'actagaaact', 'gaattttatc', 'aaagtcttag', 'agtttatata', 'tctctcatat', 'atgaaaaacc']['4981', 'acagctaaca', 'tcattcacaa', 'tggtg']['//']D:\docu\gb2fasta\gb2fasta.py目錄創建成功！你的fasta文件保存在: D:\docu\gb2fasta\singl_fasta\文件夾下程序運行前 D:\docu\gb2fasta\ 文件夾下 文件
運行前
程序運行後 D:\docu\gb2fasta\ 文件夾下 文件
運行後
程序運行後 D:\docu\gb2fasta\ singl_fasta\ 文件夾下 文件
以上就是Python實現GB格式序列文件轉換Fasta格式文件的詳細內容，更多關於Python GB轉換Fasta格式文件的資料請關注軟件開發網其它相關文章！
